![]() *See main Ion-Exchange Chromatography page for detailed description. We develop, manufacture and sell chromatography detectors and detector/gradient pump systems for FPLC, LPLC and MPLC. REACH Devices does not manufacture, does not distribute, and does not promote any ion-exchange resins. List of Commercially Available Strong Cation Exchangers * Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.Protein Purification by Ion-Exchange Chromatography Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Large “throughpores” allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short “diffusive” pores inside. In contrast to conventional chromatography media, POROS™ High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. High capacity, high resolution, high speed By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential. Both enzymes and affinity ligands can be immobilized on POROS media. High Performance Chromatography technology is not limited to standard modes of separation. ![]() Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.Ĭreate novel assays for more efficient analysis For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. You can replace many sample preparation steps with high speed High Performance Chromatography technology.
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